Method Development and Validation of Spectrophotometric Method for the Estimation of Labetalol Hydrochloride in Pure

INTRODUCTION Labetalol HCl is a selective α1 and non-selective β adrenergic blocker used to treat high blood pressure. Chemically it is 2-hydroxy-5-{[1-hydroxy-2-(4 phenyl butane -2-lyl) amino]ethyl}benzamide. It has a molecular formula of C19H24N2O3.Hcl and a molecular weight of 328.40 g/mol and its structure was given in Figure No. 1. Literature survey revealed, few analytical methods which include A Validated stability indicating HPTLC method for determination of labetalol HCl in combined dosage forms, Determination of Labetalol HCl by chromatography, Stability indicating HPTLC ABSTRACT A simple, accurate, precise and sensitive spectroscopic method was developed for the estimation of Labetalol hydrochloride in the pure and tablet dosage forms. The estimation of Labetalol hydrochloride was carried out at the maximum absorbance at 246 nm. The method was found to be linear and obeys beers law in the concentration range 1-10μg/ml with a correlation coefficient 0.999. The developed method was validated as per ICH guidelines and was found to be accurate and precise. Thus the proposed method can be successfully applied for the estimation of labetalol hydrochloride in pure and tablet dosage form.